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Chemiluminescence differs from fluorescence or phosphorescence in that the electronic excited state is the product of a chemical reaction rather than of the absorption of a photon. It is the antithesis of a photochemical reaction, in which light is used to drive an endothermic chemical reaction.
Chemiluminescence is the emission of light through a chemical reaction. It contrasts with fluorescence , which is excited by a light source. During chemiluminescence, the vibrationally excited product of an exoergic chemical reaction relaxes to its ground state with the emission of photons . [ 1 ]
Electrochemiluminescence or electrogenerated chemiluminescence (ECL) is a kind of luminescence produced during electrochemical reactions in solutions. In electrogenerated chemiluminescence, electrochemically generated intermediates undergo a highly exergonic reaction to produce an electronically excited state that then emits light upon relaxation to a lower-level state.
Chemiluminescence of luminol. To exhibit its luminescence, the luminol must be activated with an oxidant. Usually, a solution containing hydrogen peroxide (H 2 O 2) and hydroxide ions in water is the activator. In the presence of a catalyst such as an iron or periodate compound, the hydrogen peroxide decomposes to form oxygen and water: 2 H 2 O ...
Chemiluminescent immunoassay (CLIA) is a type of immunoassay employing chemiluminescence. [1] [2] See also. Enzyme-linked immunosorbent assay (ELISA) References
Luminol and haemoglobin, an example of chemiluminescence UV-photoluminescence in microbiological diagnostics. Luminescence is a spontaneous emission of radiation from an electronically or vibrationally excited species not in thermal equilibrium with its environment.
However, in the presence of a fluorescer, light can be generated (chemiluminescence). Peroxyoxalate chemiluminescence (CL) was first reported by Rauhut in 1967 [1] in the reaction of diphenyl oxalate. The emission is generated by the reaction of an oxalate ester with hydrogen peroxide in the presence of a suitably fluorescent energy acceptor.
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