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In the tissue microarray technique, a hollow needle is used to remove tissue cores as small as 0.6 mm in diameter from regions of interest in paraffin-embedded tissues such as clinical biopsies or tumor samples. These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern.
Paraffin tissue array was developed during late years in the 1980s; this array can help scientists high throughput analyze gene and protein expressions in multiple tissue samples, especially analyze different protein levels with antibodies by immunohistochemistry. Various paraffin tissue arrays are now commercially available from many biotech ...
The laser microtome has the ability to slice almost every tissue in its native state. Depending on the material being processed, slice thicknesses of 10 to 100 μm are feasible. Sectioning intervals can be classified mainly into either: Serial sectioning: obtaining a continuous ribbon of sections from a paraffin block and using all for slides.
The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer
The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. [2] The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing. While diagnosis can be rendered in many cases, fixed tissue processing is preferred in many conditions for more accurate ...
Soft tissue and bone strengthen with exercise and weaken if it is not actively used. [11] [12] Horses that undergo long-term stall rest require a slow, progressive rehabilitation program to try to prevent re-injury of the original tissue or injury to a new area now weakened by prolonged disuse. In some cases of soft tissue injury, low-impact ...
The organism is commonly found in dry and dusty soil and can be important for diseases of domesticated animals (horses and goats). The frequency of infection can reach near 60%. [1] R. equi is an important pathogen causing pneumonia in foals. Since 2008, R. equi has been known to infect wild boar and domestic pigs. [2]
The sample of tissue is immersed in fixative solution for a set period of time. The fixative solution must have a volume at least 10 times greater than the volume of the tissue. [6] In order for fixation to be successful, the fixative must diffuse throughout the entire tissue, so tissue size and density, as well as type of fixative must be ...