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Calcium imaging is a microscopy technique to optically measure the calcium (Ca 2+) status of an isolated cell, tissue or medium.Calcium imaging takes advantage of calcium indicators, fluorescent molecules that respond to the binding of Ca 2+ ions by fluorescence properties.
A chloridometer is a measuring instrument used to determine the concentration of chloride ions (Cl –) in a solution.It uses a process known as coulometric titration or amperostatic coulometry, the accepted electrochemistry reference method to determine the concentration of chloride in biological fluids, including blood serum, blood plasma, urine, sweat, and cerebrospinal fluid.
Calcium(I) chloride (CaCl) is a diatomic molecule observed in certain gases. [ 1 ] A solid with the composition CaCl was reported in 1953; [ 2 ] however, later efforts to reproduce this work failed. [ 3 ]
Calcium chloride was apparently discovered in the 15th century but wasn't studied properly until the 18th century. [11] It was historically called "fixed sal ammoniac" (Latin: sal ammoniacum fixum [12]) because it was synthesized during the distillation of ammonium chloride with lime and was nonvolatile (while the former appeared to sublime); in more modern times (18th-19th cc.) it was called ...
An assay is a type of biological titration used to determine the concentration of a virus or bacterium. Serial dilutions are performed on a sample in a fixed ratio (such as 1:1, 1:2, 1:4, 1:8, etc.) until the last dilution does not give a positive test for the presence of the virus.
The Calcium-Dependent Chloride Channel (Ca-ClC) proteins (or calcium-activated chloride channels (CaCCs), [2] are heterogeneous groups of ligand-gated ion channels for chloride that have been identified in many epithelial and endothelial cell types as well as in smooth muscle cells.
A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. [1] A detection method is used to determine the presence and amount of the ligand-receptor complexes formed, and this is usually determined electrochemically or through a fluorescence detection method. [2]
Calcium chloride treatment is generally used for the transformation of E. coli and other bacteria. [11] It enhances plasmid DNA incorporation by the bacterial cell, promoting genetic transformation. Plasmid DNA can attach to LPS by being added to the cell solution together with CaCl 2. [12]