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  2. Prokaryotic DNA replication - Wikipedia

    en.wikipedia.org/wiki/Prokaryotic_DNA_replication

    Prokaryotic DNA Replication is the process by which a prokaryote duplicates its DNA into another copy that is passed on to daughter cells. [1] Although it is often studied in the model organism E. coli, other bacteria show many similarities. [2] Replication is bi-directional and originates at a single origin of replication (OriC). [3]

  3. Escherichia coli in molecular biology - Wikipedia

    en.wikipedia.org/wiki/Escherichia_coli_in...

    E. coli colonies containing the fluorescent pGLO plasmid. Escherichia coli (/ ˌ ɛ ʃ ɪ ˈ r ɪ k i ə ˈ k oʊ l aɪ /; commonly abbreviated E. coli) is a Gram-negative gammaproteobacterium commonly found in the lower intestine of warm-blooded organisms (endotherms). The descendants of two isolates, K-12 and B strain, are used routinely in ...

  4. Circular chromosome - Wikipedia

    en.wikipedia.org/wiki/Circular_chromosome

    See Figure 4 of D. M. Prescott, and P. L. Kuempel (1972): A grain track produced by an E. coli chromosome from cells labeled for 19 min with [3H] thymine, followed by labeling for 2.5 min with [3H]thymine and ['H]thymidine. . The E. coli DNA polymerase III holoenzyme is a 900 kD complex, possessing an essentially a dimeric structure.

  5. Replication terminator Tus family - Wikipedia

    en.wikipedia.org/wiki/Replication_terminator_Tus...

    Tus helps end DNA replication in prokaryotes. [2] In E. coli, Tus binds to 10 closely related sites encoded in the chromosome. Each site is 23 base pairs. The 10 sites are called Ter sites, and are designated TerA, TerB, ..., TerJ. These binding sites are asymmetric, such that when a Tus-Ter complex (Tus protein bound to a Ter site) is ...

  6. Escherichia coli - Wikipedia

    en.wikipedia.org/wiki/Escherichia_coli

    E. coli is a chemoheterotroph whose chemically defined medium must include a source of carbon and energy. [16] E. coli is the most widely studied prokaryotic model organism, and an important species in the fields of biotechnology and microbiology, where it has served as the host organism for the majority of work with recombinant DNA. Under ...

  7. DNA mismatch repair - Wikipedia

    en.wikipedia.org/wiki/DNA_mismatch_repair

    Mismatch repair is a highly conserved process from prokaryotes to eukaryotes. The first evidence for mismatch repair was obtained from S. pneumoniae (the hexA and hexB genes). Subsequent work on E. coli has identified a number of genes that, when mutationally inactivated, cause hypermutable strains. The gene products are, therefore, called the ...

  8. Shuttle vector - Wikipedia

    en.wikipedia.org/wiki/Shuttle_vector

    Shuttle vectors include plasmids that can propagate in eukaryotes and prokaryotes (e.g. both Saccharomyces cerevisiae and Escherichia coli) or in different species of bacteria (e.g. both E. coli and Rhodococcus erythropolis). There are also adenovirus shuttle vectors, which can propagate in E. coli and mammals.

  9. DNA polymerase I - Wikipedia

    en.wikipedia.org/wiki/DNA_polymerase_I

    It was initially characterized in E. coli and is ubiquitous in prokaryotes. In E. coli and many other bacteria, the gene that encodes Pol I is known as polA . The E. coli Pol I enzyme is composed of 928 amino acids, and is an example of a processive enzyme — it can sequentially catalyze multiple polymerisation steps without releasing the ...