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The most common cryoprotectant used for semen is glycerol (10% in culture medium). Often sucrose or other di-, trisaccharides are added to glycerol solution. Cryoprotectant media may be supplemented with either egg yolk or soy lecithin, with the two having no statistically significant differences compared to each other regarding motility, morphology, ability to bind to hyaluronate in vitro, or ...
Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA fragmentation. [1] First described by Evenson in 1980, the assay is a flow cytometric test that detects the vulnerability of sperm DNA to acid-induced denaturation DNA in situ. [2]
How long the man has abstained prior to providing a semen sample correlates with the results of semen analysis and also with success rates in assisted reproductive technology (ART). Both a too short period of time since last ejaculation and a too long one reduce semen quality. A period of time of less than one day reduces sperm count by at ...
A semen analysis (plural: semen analyses), also called seminogram or spermiogram, evaluates certain characteristics of a male's semen and the sperm contained therein. [1] [2] [3] It is done to help evaluate male fertility, whether for those seeking pregnancy or verifying the success of vasectomy. Depending on the measurement method, just a few ...
A meta-analysis found no evidence that high doses of lecithin improved milk flow in breast-feeding mothers or infants, though concluded that "higher maternal choline intake was likely to be associated with better child neurocognition and neurodevelopment." [25] [20]
Semen sample obtaining is the first step in spermiogram. The optimal sexual abstinence for semen sample obtaining is of 2–7 days. The first way to obtain the semen sample is through masturbation, and the best place to obtain it is in the same clinic, as this way temperature changes during transport can be avoided, which can be lethal for some ...
One main test that is performed on amniotic fluid is the L/S ratio test (lecithin/sphingomyelin). This test is used to determine fetal lung maturity. Both lecithin and sphingomyelin are lung surfactants that are present in increasing amounts in the maturing fetus, though past week 33, sphingomyelin levels remain relatively constant.
Introducing semen into the vaginal cavity during the ovulation time, called 'timing of intercourse' or 'timing of artificial insemination', is important for achieving a pregnancy. Where a donor is being used, timing is particularly important because donor sperm which is supplied by a sperm bank or a fertility clinic is expensive and it is ...