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  2. Isopropyl β-D-1-thiogalactopyranoside - Wikipedia

    en.wikipedia.org/wiki/Isopropyl_β-D-1...

    Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .

  3. T7 expression system - Wikipedia

    en.wikipedia.org/wiki/T7_expression_system

    IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]

  4. β-Galactosidase - Wikipedia

    en.wikipedia.org/wiki/Β-Galactosidase

    Beta-gal is inhibited by L-ribose and by competitive inhibitors 2-phenylethyl 1-thio-β-D-galactopyranoside (PETG), D-galactonolactone, isopropyl thio-β-D-galactoside (IPTG), and galactose. [ 4 ] β-Galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of ...

  5. Synthetic biological circuit - Wikipedia

    en.wikipedia.org/wiki/Synthetic_biological_circuit

    IPTG, a molecule similar to lactose, but with a sulfur bond that is not hydrolyzable so that E. coli does not digest it, is used to activate or "induce" the production of the new protein. Once the cells are induced, it is difficult to remove IPTG from the cells and therefore it is difficult to stop expression.

  6. Tac-Promoter - Wikipedia

    en.wikipedia.org/wiki/Tac-Promoter

    The expression of PTAC is repressed by the lacI protein. The lacI q allele is a promoter mutation that increases the intracellular concentration of LacI repressor, resulting in the strong repression of PTAC. The addition of the inducer IPTG inactivates the LacI repressor.

  7. This Baltimore job hunter avoided an employment scam by ...

    www.aol.com/finance/baltimore-job-hunter-avoided...

    Since the pandemic, a lot of companies have made a shift to remote work. Many employees have found that working from home offers a lot of benefits, including saving on transportation costs.

  8. It's time for Nordstrom's Half-Yearly Sale: Stock up on ... - AOL

    www.aol.com/lifestyle/its-time-for-nordstroms...

    Sure, Dyson's dryers get all the attention, but they're not the only professional-grade powerhouses out there. This T3 model is designed to treat every hair type with minimal damage and includes ...

  9. Inducer - Wikipedia

    en.wikipedia.org/wiki/Inducer

    Index inducer or just inducer predictably induce metabolism via a given pathway and are commonly used in prospective clinical drug-drug interaction studies. [ 4 ] Strong, moderate, and weak inducers are drugs that decreases the AUC of sensitive index substrates of a given metabolic pathway by ≥80%, ≥50% to <80%, and ≥20% to <50% ...