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The fungi Saccharomyces produces ascospores when grown on V-8 medium, acetate ascospore agar, or Gorodkowa medium. These ascospores are globose and located in asci. Each ascus contains one to four ascospores. The asci do not rupture at maturity. Ascospores are stained with Kinyoun stain and ascospore stain.
An apothecium (plural: apothecia) is a wide, open, saucer-shaped or cup-shaped fruit body. It is sessile and fleshy. The structure of the apothecium chiefly consists of three parts: hymenium (upper concave surface), hypothecium, and excipulum (the "foot"). The asci are present in the hymenium layer. The asci are freely exposed at maturity.
Peziza cerea, commonly known as the Cellar Cup is a species of ascomycete fungus in the genus Peziza, family Pezizaceae.In common with other ascomycetes the upper surface of the fungus has a layer of cylindrical spore producing cells called asci, from which the ascospores are forcibly discharged.
The structure of the ascospores is the primary characteristic separating genus Bruceomyces from Resinogalea. In the former, they are broadly ellipsoidal with distinct surface ornamentations, while in the latter they are smooth with a biconcave disc structure similar to that of red blood cells .
The defining feature of this fungal group is the "ascus" (from Ancient Greek ἀσκός (askós) 'sac, wineskin'), a microscopic sexual structure in which nonmotile spores, called ascospores, are formed. However, some species of Ascomycota are asexual and thus do not form asci or ascospores.
The bitunicate asci are thick-walled, with an outer and inner layer. The outer and inner layer of the ascus wall are called exotunica and endotunica, and they separate during ascospore release. [6] The asci usually contains eight ascospores, while the shape and separation of the ascospores are more variating. [13]
The clavate asci which contain ascospores are produced by the ascogenous hyphae with the broad base or a short broad stalk. [1] Only a cluster of asci would be formed in a mature apothecium, and as the asci successfully mature, the asci shrink to a short stipe and give rise to a large attached operculum . [ 8 ]
The asci elongate into the ostiole, and discharge the ascospores outward. The spore distribution is a lengthy process, sometimes taking several months to complete. The spore print is black. [3] In springtime this fungus often produces a layer of white or bluish asexual spores called conidia, which grow on its surface and surrounding area. [4]