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Figure 1: Growth curve for cells grown in culture. Cells should be subcultured while still in the exponential phase. Calculate the population doubling time, or the time required for a culture to double in number, with the following formula: DT=T ln2/ln(Xe/Xb) T is the incubation time in any units.
This entailed investigating conditions affecting growth, metabolism, and phenotype dynamics to ensure cell quality is appropriate for use. Results revealed critical process parameters (CPPs) including feeding regime and seeding density impact critical quality attributes (CQAs) including specific metabolic rate (SMR) and specific growth rate (SGR).
Cell growth (proliferation) can be evaluated by the time-dependent changes of the total number of proliferating cells, as well as the ratio of cells among individual phases of the cell cycle. The cell growth curve was plotted to show the time-dependent increase in cell numbers.
A type of mathematic function called a growth curve describes increases and decreases in the number of cells over time. These curves not only summarize clinical course, but they also relate to the rate of emergence of mutations toward clinically relevant cellular diversity.
A growth curve is an empirical model of the evolution of a quantity over time. Growth curves are widely used in biology for quantities such as population size or biomass (in population ecology and demography, for population growth analysis), individual body height or biomass (in physiology, for growth analysis of individuals).
Modeling cell population growth. (A) Exponential growth, logistic growth, and the Allee effect. (B) Growth curves for the Baranyi model.
The cell number (red lines) and cell mass (blue lines) growth curves formed two parallel lines in semi-log plots, indicating the steady-state growth had been achieved.
The classic growth curve, as exemplified by a newly established bacterial colony, is divided into four phases, in order of their appearance: (1) lag phase; (2) log (logarithmic), or exponential, phase; (3) stationary phase; and (4) death, or decline, phase.
Growth curves. Top graph shows the rate of growth plotted on a linear scale. Bottom graph shows the same data plotted on a log scale. Although cells can grow indefinitely if given the space, labs cannot afford to work with flasks that are too large. Therefore cells are often grown in a series of dilutions.
Studies of single-celled yeast and bacteria have revealed that in order to achieve size homeostasis, cells must modulate the amount of growth produced during the cell cycle such that, on...