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The latter prevents cross-contamination and stationary phase degradation due to recycling. Column chromatography can be done using gravity to move the solvent, or using compressed gas to push the solvent through the column. A thin-layer chromatograph can show how a mixture of compounds will behave when purified by column chromatography. The ...
Chromatography employs continuous adsorption and desorption on a packed bed of a solid to purify multiple components of a single feed stream. In a laboratory setting, mixture of dissolved materials are typically fed using a solvent into a column packed with an appropriate adsorbent, and due to different affinities for solvent (moving phase ...
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the stationary phase is fixed ...
All chromatographic purifications and separations which are executed via solvent gradient batch chromatography can be performed using MCSGP. Typical examples are reversed phase purification of peptides, hydrophobic interaction chromatography for fatty acids or for example ion exchange chromatography of proteins or antibodies. The process can ...
Elution principle of column chromatography. In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange resins to remove captured ions, or eluting proteins or other biopolymers from a gel electrophoresis or chromatography column.
The two components of the mobile phase are typically termed "A" and "B"; A is the "weak" solvent which allows the solute to elute only slowly, while B is the "strong" solvent which rapidly elutes the solutes from the column. In reversed-phase chromatography, solvent A is often water or an aqueous buffer, while B is an organic solvent miscible ...
The distribution coefficient (K c which coincides with partition coefficient) of atabrine varied by the composition of the solvent system and the pH of the buffer. In the next article, Craig was inspired by the work of Martin and Synge with partition chromatography to develop an apparatus that would separate compounds based on their ...
However, chromatography columns with an inner diameter (ID) of up to 5 cm are generally considered small scale or laboratory scale columns. Small scale chromatography columns are mostly intended for design of experiments (DoE); proof of concept; validation (drug manufacture) or research and development experiments. Columns of this scale ...