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Initiation of translation in bacteria involves the assembly of the components of the translation system, which are: the two ribosomal subunits (50S and 30S subunits); the mature mRNA to be translated; the tRNA charged with N-formylmethionine (the first amino acid in the nascent peptide); guanosine triphosphate (GTP) as a source of energy, and the three prokaryotic initiation factors IF1, IF2 ...
Prokaryotic translation may refer to: Bacterial translation, the process by which messenger RNA is translated into proteins in bacteria; Archaeal translation, the ...
While prokaryotes are able to undergo both cellular processes simultaneously, the spatial separation that is provided by the nuclear membrane prevents this coupling in eukaryotes. Eukaryotic elongation factor 2 (eEF2) is a regulateable GTP -dependent translocase that moves nascent polypeptide chains from the A-site to the P-site in the ribosome.
The strict regulation of translation in both space and time is in part governed by cis-regulatory elements located in 5′ mRNA transcript leaders (TLs) and 3′ untranslated regions (UTRs). Due to their role in translation initiation, mRNA 5′ transcript leaders (TLs) strongly influence protein expression.
In prokaryotes (bacteria and archaea), translation occurs in the cytosol, where the large and small subunits of the ribosome bind to the mRNA. In eukaryotes , translation occurs in the cytoplasm or across the membrane of the endoplasmic reticulum through a process called co-translational translocation .
Degradation of prokaryotic mRNAs is accelerated by loss of coupled translation due to increased availability of target sites of RNase E. [6] It has also been suggested that coupling of transcription with translation is an important mechanism of preventing formation of deleterious R-loops . [ 7 ]
Phosphorylation is highly effective for controlling the enzyme activity and is the most common change after translation. [ 2 ] Many eukaryotic and prokaryotic proteins also have carbohydrate molecules attached to them in a process called glycosylation , which can promote protein folding and improve stability as well as serving regulatory functions.
The translocon (also known as a translocator or translocation channel) is a complex of proteins associated with the translocation of polypeptides across membranes. [1] In eukaryotes the term translocon most commonly refers to the complex that transports nascent polypeptides with a targeting signal sequence into the interior (cisternal or lumenal) space of the endoplasmic reticulum (ER) from ...