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Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton, who were microbiologists working at Harvard University.
Subsequently, aliquots of the sample are taken to test for the antimicrobial activity of a specific drug or protein (antimicrobial peptides). Liquid cultures of the cyanobacterium Synechococcus PCC 7002. Static liquid cultures may be used as an alternative. These cultures are not shaken, and they provide the microbes with an oxygen gradient. [6]
An example of such testing is antibiotic susceptibility testing by measurement of minimum inhibitory concentration which is routinely used in medical microbiology and research. If a suspension used is too heavy or too dilute, an erroneous result (either falsely resistant or falsely susceptible) for any given antimicrobial agent could occur.
Microbiology (from Ancient Greek μῑκρος (mīkros) 'small' βίος (bíos) 'life' and -λογία 'study of') is the scientific study of microorganisms, those being of unicellular (single-celled), multicellular (consisting of complex cells), or acellular (lacking cells).
Once a bacterium has been identified following microbiological culture, antibiotics are selected for susceptibility testing. [5] Susceptibility testing methods are based on exposing bacteria to antibiotics and observing the effect on the growth of the bacteria (phenotypic testing), or identifying specific genetic markers (genetic testing). [6]
Koch, a pioneer in medical microbiology, worked on cholera, anthrax and tuberculosis. In his research into tuberculosis Koch finally proved the germ theory, for which he received a Nobel Prize in 1905. [5] In Koch's postulates, he set out criteria to test if an organism is the cause of a disease, and these postulates are still used today.
Medical microbiology, the large subset of microbiology that is applied to medicine, is a branch of medical science concerned with the prevention, diagnosis and treatment of infectious diseases. In addition, this field of science studies various clinical applications of microbes for the improvement of health.
Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, note that the non-selective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other than those of Neisseria gonorrhoeae, while the selective Thayer–Martin medium on the right, containing antimicrobials that inhibit the growth of ...