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The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
The plates are left upright on the bench to dry before inversion and incubation at 37 °C for 18 – 24 hours (or appropriate incubation conditions considering the organism and agar used). Each sector is observed for growth, high concentrations will give a confluent growth over the area of the drop, or a large number of small/merged colonies.
Before Balitbangkes existed, the agency was preceded by four Health Laboratories under the Indonesian Department of Health formed in 1950-1960s, three were soon founded after the end of Indonesian National Revolution: Institute for People's Food in Bogor (researched human nutrition), Central Institute for Investigation and Eradication of Venereal Diseases in Surabaya (researched transmission ...
The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been covered. The loop then is re-sterilized and the plate is turned 90 degrees. Starting in the previously streaked section, the loop is dragged through it two to three times continuing the zigzag pattern.
The sample is then cooled and inspected for pour point as per the usual pour point method. The method usually gives higher pour point because the thermal history has not been cancelled by a prolonged thermal treatment. The lower pour point is measured by first pouring the sample into a stainless steel pressure vessel. The vessel is then screwed ...
The plate theory attributes all volcanic activity on Earth, even that which appears superficially to be anomalous, to the operation of plate tectonics. According to the plate theory, the principal cause of volcanism is extension of the lithosphere. Extension of the lithosphere is a function of the lithospheric stress field. The global ...
Cup plate made of pressed glass. Cup plates are coasters that provide a place to rest a tea cup while leaving space for a light snack. Teacup plates originated in England in the early 1800s and went out of fashion in the second half of the 19th century [1] (Barber puts the peak of popularity in the US at 1840s [2]), with a brief reappearance in the first third of the 20th century as bridge ...