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The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar. Incubation of the plates is the next step and is carried out for about 3 days at 20 to 30 degrees Celsius.
Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the optimal temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius, or the human body temperature, for cultures from humans or animals, or lower for environmental cultures). After the desired ...
The sealed anaerobic jar is then incubated at a desired temperature to allow growth of the bacteria. In the above figure for example, the incubation occurred at 100 °F (38 °C). A colorless indicator strip provides proof that the anaerobic conditions were met and the agar plates can now be observed for bacterial growth.
For some water systems a weekly dip slide test is recommended. For multiple tests the incubation period and temperature should be the same each time a new sample is assessed. Bacteria present in the sample liquid will grow and form colonies. A bacterial reference chart is used to determine the number of bacteria in the sample.
R2A agar (Reasoner's 2A agar) is a culture medium [1] developed to study bacteria which normally inhabit potable water. [2] These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium.
The incubator maintains optimal temperature, humidity and other conditions such as the CO 2 and oxygen content of the atmosphere inside. Incubators are essential for much experimental work in cell biology , microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells.
At the end of incubation there should be enough bacteria to form visible colonies in the areas touched by the inoculation loop. From these mixed colonies, single bacterial or fungal species can be identified based on their morphological (size/shape/colour) differences, and then sub-cultured to a new media plate to yield a pure culture for ...
Most water baths have a digital or an analogue interface to allow users to set a desired temperature, but some water baths have their temperature controlled by a current passing through a reader. Uses include warming of reagents, melting of substrates, determination of boiling point, or incubation of cell cultures. It is also used to enable ...