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Diagram of a cephalopod's photophore, in vertical section. A photophore is a glandular organ that appears as luminous spots on marine animals, including fish and cephalopods. The organ can be simple, or as complex as the human eye, equipped with lenses, shutters, color filters, and reflectors; unlike an eye, however, it is optimized to produce ...
Stimulated emission depletion is a simple example of how higher resolution surpassing the diffraction limit is possible, but it has major limitations. STED is a fluorescence microscopy technique which uses a combination of light pulses to induce fluorescence in a small sub-population of fluorescent molecules in a sample.
A diffraction pattern of a red laser beam projected onto a plate after passing through a small circular aperture in another plate. Diffraction is the interference or bending of waves around the corners of an obstacle or through an aperture into the region of geometrical shadow of the obstacle/aperture.
In a digital camera, diffraction effects interact with the effects of the regular pixel grid. The combined effect of the different parts of an optical system is determined by the convolution of the point spread functions (PSF). The point spread function of a diffraction limited circular-aperture lens is simply the Airy disk. The point spread ...
A common optical phenomenon involving water droplets is the glory. [23] A glory is an optical phenomenon, appearing much like an iconic Saint's halo about the head of the observer, produced by light backscattered (a combination of diffraction, reflection and refraction) towards its source by a cloud of uniformly sized water droplets. A glory ...
Physical optics is used to explain effects such as diffraction. In physics, physical optics, or wave optics, is the branch of optics that studies interference, diffraction, polarization, and other phenomena for which the ray approximation of geometric optics is not valid.
Oil immersion objective lenses look superficially identical to non-oil immersion lenses. In light microscopy , oil immersion is a technique used to increase the resolving power of a microscope . This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index , thereby increasing the numerical ...
Because diffraction is the result of addition of all waves (of given wavelength) along all unobstructed paths, the usual procedure is to consider the contribution of an infinitesimally small neighborhood around a certain path (this contribution is usually called a wavelet) and then integrate over all paths (= add all wavelets) from the source to the detector (or given point on a screen).