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Knowing the structure of a similar homologous sequence (for example a member of the same protein family) allows highly accurate prediction of the tertiary structure by homology modeling. If the full-length protein sequence is available, it is possible to estimate its general biophysical properties , such as its isoelectric point .
In 1885, Theodor Escherich, a German pediatrician, first discovered this species in the feces of healthy individuals and called it Bacterium coli commune because it is found in the colon and early classifications of Prokaryotes placed these in a handful of genera based on their shape and motility (at that time Ernst Haeckel's classification of Bacteria in the kingdom Monera was in place [3]).
Therefore, the pair of a and c have the desired property, showing that this partial order obeys the 1/3–2/3 conjecture. This example shows that the constants 1/3 and 2/3 in the conjecture are tight; if q is any fraction strictly between 1/3 and 2/3, then there would not exist a pair x, y in which x is earlier than y in a number of partial ...
Lactic acid bacteria (LAB) already exists as part of the natural flora in most vegetables. Lettuce and cabbage were examined to determine the types of lactic acid bacteria that exist in the leaves. Different types of LAB will produce different types of silage fermentation, which is the fermentation of the leafy foliage. [ 19 ]
Mark and recapture is a method commonly used in ecology to estimate an animal population's size where it is impractical to count every individual. [1] A portion of the population is captured, marked, and released. Later, another portion will be captured and the number of marked individuals within the sample is counted.
The discovery of DNA ligase dates back to 1967 and was an important event in the field of molecular biology. [1] Ligation in the laboratory is normally performed using T4 DNA ligase. It is broadly used in vitro due to its capability of joining sticky-ended fragments as well as blunt-ended fragments. [2]
A master mix is a mixture containing precursors and enzymes used as an ingredient in polymerase chain reaction techniques in molecular biology. Such mixtures contain a mixture dNTPs (required as a substrate for the building of new DNA strands), MgCl 2, Taq polymerase (an enzyme required to building new DNA strands), a pH buffer and come mixed in nuclease-free water.
This is a quantification of how many cells were altered by 1 μg of plasmid DNA. In essence, it is a sign that the transformation experiment was successful. [1] It should be determined under conditions of cell excess. [2] Transformation efficiency is typically measured as the number of transformed cells per total number of cells.