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Resuspend in cold CaCl 2 as in step 4; Pour off supernatant and resuspend cells in 5 mL ice-cold 0.1 M CaCl 2 along with 15% glycerol to combine pellets; Transfer the suspensions to sterile thin glass tubes for effective heat shocks; Add the required mg amount of DNA in the suspension tubes, and immediately leave on ice
This glossary of biology terms is a list of definitions of fundamental terms and concepts used in biology, the study of life and of living organisms.It is intended as introductory material for novices; for more specific and technical definitions from sub-disciplines and related fields, see Glossary of cell biology, Glossary of genetics, Glossary of evolutionary biology, Glossary of ecology ...
Meaning, suspension cells can exist in much larger quantities in a given flask and are preferred when using cells to make products including proteins, antibodies, metabolites or just to produce a high volume of cells. However, there are far fewer mammalian suspension cell lines than mammalian adhesive cell lines.
A suspension of flour mixed in a glass of water, showing the Tyndall effect. In chemistry, a suspension is a heterogeneous mixture of a fluid that contains solid particles sufficiently large for sedimentation.
nomen ambiguum (plural: nomina ambigua), (bot.) a name that has been used with more than one meaning; nomen confusum (plural: nomina confusa), (bact.) a name based on a mixed bacterial culture; nomen perplexum (plural: nomina perplexa), a name confusingly similar to another name or names
Also called functionalism. The Darwinian view that many or most physiological and behavioral traits of organisms are adaptations that have evolved for specific functions or for specific reasons (as opposed to being byproducts of the evolution of other traits, consequences of biological constraints, or the result of random variation). adaptive radiation The simultaneous or near-simultaneous ...
The community context considers a relatively constant environment in which multiple stable states are accessible to populations or communities. This definition is an extension of stability analysis of populations (e.g., Lewontin 1969; Sutherland 1973) and communities (e.g., Drake 1991; Law and Morton 1993).
In biology the term 'condensation' is used much more broadly and can also refer to liquid–liquid phase separation to form colloidal emulsions or liquid crystals within cells, and liquid–solid phase separation to form gels, [1] sols, or suspensions within cells as well as liquid-to-solid phase transitions such as DNA condensation during ...