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Cellulase (EC 3.2.1.4; systematic name 4-β-D-glucan 4-glucanohydrolase) is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related polysaccharides: Endohydrolysis of (1→4)-β-D-glucosidic linkages in cellulose, lichenin and cereal β-D-glucan
These enzymes degrade complex organic matter such as cellulose and hemicellulose into simple sugars that enzyme-producing organisms use as a source of carbon, energy, and nutrients. [2] Grouped as hydrolases , lyases , oxidoreductases and transferases , [ 1 ] these extracellular enzymes control soil enzyme activity through efficient degradation ...
Moreover, CMC has been used extensively to characterize enzyme activity from endoglucanases (part of the cellulase complex); it is a highly specific substrate for endo-acting cellulases, as its structure has been engineered to decrystallize cellulose and create amorphous sites that are ideal for endoglucanase action.
CodeXyme® 3 (Codexis' cellulase enzyme from 2011) was found to convert the same or more glucan at the same enzyme load as competing cellulase packages, and CodeXyme® 4 fared even better against ...
This enzyme catalyses the hydrolysis of (1→4)-β-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains. CBH1 from yeast, for example, is composed of a carbohydrate binding site, a linker region and a catalytic domain. [ 6 ]
The microbial degradation of cellulose and xylans requires several types of enzymes. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family A [9] or as the glycosyl hydrolases ...
Enzyme washing or "bio stoning" is a textile manufacturing technique in which cellulase enzymes are applied to denim. As with traditional stone washing, the intended effect is a faded appearance and softer feel. [1] Because the technique relies on biotechnology, enzyme washing is considered an example of textile bio-processing. [2] [3]
Fibrolytic enzymes, which are classified as cellulases, can hydrolyze the β (1 ->4) bonds in plant polysaccharides. Cellulase and hemicellulase (also known as xylanase) are the two main representatives of these enzymes.