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A mix-up rated as contamination could in reality be a simple confusion of two cell lines, but usually contamination is assumed. [citation needed] After a cell line has been discovered to be contaminated, it is usually never used again for research demanding the specific type of cell line they were assumed to be.
Contamination on agar plate. In the biological sciences, accidental introduction of "foreign" material can seriously distort the results of experiments where small samples are used. In cases where the contaminant is a living microorganism, it can often multiply to dominate the sample and render it useless, as in contaminated cell culture lines.
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Whole Foods may end up cross-contaminating other stores with the labeling system, drawing even more attention to the company's large selection of health-conscious -- and high-margin -- products.
HeLa cells are rapidly dividing cancer cells, and the number of chromosomes varies during cancer formation and cell culture. The current estimate (excluding very tiny fragments) is a "hypertriploid chromosome number (3n+)", which means 76 to 80 total chromosomes (rather than the normal diploid number of 46) with 22–25 clonally abnormal ...
A cell line can be defined as a permanently established cell culture which will propagate forever. Investigators mostly get cell lines from other investigators or from cell banks (such as the American Type Culture Collection) , because its much easier than creating new one. In special cases, investigators are obligated to establish a cell line.
Once a line is generated, all progeny will be germ-free unless contaminated. These progeny can then be used for experimentation. Typically for experiments, each mouse is housed separately in a sterile isolator to prevent cross-contamination between mice. The mice are provided with sterilized food and water to prevent contamination.
Cell banks can be used to generate detailed characterizations of cell lines and can also help mitigate cross-contamination of a cell line. [1] Utilizing cell banks also reduces the cost of cell culture processes, providing a cost-efficient alternative to keeping cells in culture constantly.