Search results
Results from the WOW.Com Content Network
A polyhistidine-tag, best known by the trademarked name His-tag, is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as a hexa histidine-tag, 6xHis-tag, or His6 tag.
Histidine ball and stick model spinning. Histidine (symbol His or H) [2] is an essential amino acid that is used in the biosynthesis of proteins.It contains an α-amino group (which is in the protonated –NH 3 + form under biological conditions), a carboxylic acid group (which is in the deprotonated –COO − form under biological conditions), and an imidazole side chain (which is partially ...
In orthodox two-component signaling, a histidine kinase protein autophosphorylates on a histidine residue in response to an extracellular signal, and the phosphoryl group is subsequently transferred to an aspartate residue on the receiver domain of a response regulator. In phosphorelays, the "hybrid" histidine kinase contains an internal ...
^a CID 71083 from PubChem (D-histidine) ^a CID 6274 from PubChem (L-histidine) This page was last edited on 12 April 2023, at 11:40 (UTC). Text is ...
In enzymology, a histidine N-acetyltransferase (EC 2.3.1.33) is an enzyme that catalyzes the chemical reaction. acetyl-CoA + L-histidine CoA + N-acetyl-L-histidine. Thus, the two substrates of this enzyme are acetyl-CoA and L-histidine, whereas its two products are CoA and N-acetyl-L-histidine.
The Pauly reaction is a chemical test used for detecting the presence of tyrosine or histidine in proteins. It is named after German chemist Hermann Pauly , who first described the reaction. [ 1 ] When proteins containing either tyrosine or histidine are reacted with diazotized sulfanilic acid under alkaline conditions, a red color is formed by ...
HIS-selective medium is a type cell culture medium that lacks the amino acid histidine. It can be used with bacteria reliant on the expression of a gene encoding proteins involved in histidine expression in order to survive. [1]
In enzymology, a histidine-tRNA ligase (EC 6.1.1.21) is an enzyme that catalyzes the chemical reaction. ATP + L-histidine + tRNAHis AMP + diphosphate + L-histidyl-tRNAHis. The 3 substrates of this enzyme are ATP, L-histidine, and tRNA(His), whereas its 3 products are AMP, diphosphate, and L-histidyl-tRNA(His).