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Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
Plant development is the process by which structures originate and mature as a plant grows. It is a subject studies in plant anatomy and plant physiology as well as plant morphology. The process of development in plants is fundamentally different from that seen in vertebrate animals. When an animal embryo begins to develop, it will very early ...
Plant anatomy or phytotomy is the general term for the study of the internal structure of plants. Originally, it included plant morphology , the description of the physical form and external structure of plants, but since the mid-20th century, plant anatomy has been considered a separate field referring only to internal plant structure.
Confocal microscope images of a tomato leaf from Solanum lycopersicum. Brightfield DIC image showing guard cells and pavement cells (above). Same region showing Chlorophyll A autofluorescence with 440 nm laser excitation and far red emission (below). Microscopic images of a moss leaf from Plagiomnium undulatum.
(a) Optically sectioned fluorescence images of a pollen grain. (b) Combined image. (c) Combined image of a group of pollen grains. [1]Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample.
[1] [2] A fluorescence microscope is any microscope that uses fluorescence to generate an image, whether it is a simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image. [3]
By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...
Autofluorescence super resolution microscopy/optical nanoscopy image of cellular structures that are invisible with confocal light microscopy. In a few cases, autofluorescence may actually illuminate the structures of interest, or serve as a useful diagnostic indicator. [1]