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Optical magnification is the ratio between the apparent size of an object (or its size in an image) and its true size, and thus it is a dimensionless number. Optical magnification is sometimes referred to as "power" (for example "10× power"), although this can lead to confusion with optical power.
The actual power or magnification of a compound optical microscope is the product of the powers of the eyepiece and the objective lens. For example a 10x eyepiece magnification and a 100x objective lens magnification gives a total magnification of 1,000×.
is the total magnification; Axial optical units are more complicated, as there is no simple definition of resolution in the axial direction. There are two forms of the optical unit for the axial direction. For the case of a system with high numerical aperture, the axial optical units in a distance z are given by:
One of the most important properties of microscope objectives is their magnification.The magnification typically ranges from 4× to 100×. It is combined with the magnification of the eyepiece to determine the overall magnification of the microscope; a 4× objective with a 10× eyepiece produces an image that is 40 times the size of the object.
This definition of lens power relies upon an arbitrary decision to split the angular magnification of the instrument into separate factors for the eyepiece and the objective. Historically, Abbe described microscope eyepieces differently, in terms of angular magnification of the eyepiece and 'initial magnification' of the objective.
Bright-field microscopy is a standard light-microscopy technique, and therefore magnification is limited by the resolving power possible with the wavelength of visible light. The practical limit to magnification with a light microscope is around 1300×.
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In doing so, the overall angular magnification of the system varies, changing the effective focal length of the complete zoom lens. At each of the three points shown, the three-lens system is afocal (neither diverging or converging the light), and hence does not alter the position of the focal plane of the lens.