Search results
Results from the WOW.Com Content Network
High-throughput phenotypic testing is increasingly important for exploring the biology of bacteria, fungi, yeasts, and animal cell lines such as human cancer cells.Just as DNA microarrays and proteomic technologies have made it possible to assay the expression level of thousands of genes or proteins all a once, phenotype microarrays (PMs) make it possible to quantitatively measure thousands of ...
High-content screening where changes in the expression of several proteins can be simultaneously monitored is also often used. [9] [10] High-content imaging of dye-labeled cellular components can also reveal effects of compounds on cell cultures in vitro, distinguishing the phenotypic effects of a broad variety of drugs. [11]
The choice of a chemical library is less stringent than other high-throughput screening strategies owing to the lack of functional readouts, which would otherwise require deconvolution of the source compound that generates biological activity. Thus, the typical range for AS-MS is 400-3,000 compounds per pool. [20]
High-throughput screening (HTS) is a method for scientific discovery especially used in drug discovery and relevant to the fields of biology, materials science [1] and chemistry. [ 2 ] [ 3 ] Using robotics , data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to ...
Classical High throughput screening robotics are now being tied closer to cell biology, principally using technologies such as High-content screening.High throughput cell biology dictates methods that can take routine cell biology from low scale research to the speed and scale necessary to investigate complex systems, achieve high sample size, or efficiently screen through a collection.
Similar to classical genetic screens in the past, large-scale RNAi surveys success depends on a careful development of phenotypic assays and their interpretation. [9] In Drosophila , RNAi has been applied in cultured cells or in vivo to investigate gene functions and to effect the function of single genes on a genome-wide scale.
Using AOL Calendar lets you keep track of your schedule with just a few clicks of a mouse. While accessing your calendar online gives you instant access to appointments and events, sometimes a physical copy of your calendar is needed. To print your calendar, just use the print functionality built into your browser.
Following phenotypic selection, genomic DNA is extracted from the selected clones, alongside a control cell population. [ 23 ] [ 46 ] [ 49 ] In the most common protocols for genome-wide knockouts, a 'Next-generation sequencing (NGS) library' is created by a two step polymerase chain reaction (PCR).