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  2. Alexa Fluor - Wikipedia

    en.wikipedia.org/wiki/Alexa_Fluor

    The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology . [ 1 ]

  3. Fluorescence microscope - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_microscope

    A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, ... Alexa 488, Alexa 568. LIMON microscopy.

  4. Vertico spatially modulated illumination - Wikipedia

    en.wikipedia.org/wiki/Vertico_spatially...

    3D dual colour super resolution microscopy with Her2 and Her3 in breast cancer cells, standard dyes: Alexa 488, Alexa 568 - LIMON (SPDM +SMI. Vertico spatially modulated illumination (Vertico-SMI) is the fastest [citation needed] light microscope for the 3D analysis of complete cells in the nanometer range.

  5. Immunofluorescence - Wikipedia

    en.wikipedia.org/wiki/Immunofluorescence

    Green = smooth muscle actin (SMA) with Alexa 488 fluorophore. Blue = DAPI counterstain. Red = auto-fluorescence. Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level.

  6. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    Many standard fluorescent dyes like GFP, Alexa dyes, Atto dyes, Cy2/Cy3 and fluorescein molecules can be used for localization microscopy, provided certain photo-physical conditions are present. Using this so-called SPDMphymod (physically modifiable fluorophores) technology a single laser wavelength of suitable intensity is sufficient for ...

  7. Super-resolution microscopy - Wikipedia

    en.wikipedia.org/wiki/Super-resolution_microscopy

    A 4Pi microscope is a laser-scanning fluorescence microscope with an improved axial resolution. The typical value of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy.

  8. Fluorescence correlation spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_correlation...

    Light sheet fluorescence microscopy or selective plane imaging microscopy (SPIM) uses illumination that is done perpendicularly to the direction of observation, by using a thin sheet of (laser) light. Under certain conditions, this illumination principle can be combined with fluorescence correlation spectroscopy, to allow spatially resolved ...

  9. Rhodamine - Wikipedia

    en.wikipedia.org/wiki/Rhodamine

    Other derivatives of rhodamine include newer fluorophores such as Alexa 546, Alexa 633, DyLight 550 and DyLight 633, HiLyte fluor 555 HiLyte 594, Janelia Dyes JF549 and JF669 have been tailored for various chemical and biological applications where higher photostability, increased brightness, different spectral characteristics, or different ...

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