Search results
Results from the WOW.Com Content Network
The smear is first treated with chloroform to remove fats . Smear applied with Alberts stain which contains cationic dyes such as toluidine blue and malachite green. Toluidine blue preferentially stains granules while malachite green stains cytoplasm. The granules show the typical monochromatism nature, this is used to demonstrate granules
This diluted bacteria sample is commonly referred to as a smear after it is placed on a slide. After a smear has dried at room temperature, the slide is gripped by tongs or a clothespin and passed through the flame of a Bunsen burner several times to heat-kill and adhere the organism to
Applying a primary stain (crystal violet) to a heat-fixed smear of a bacterial culture. Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they do not rinse out during the staining procedure. The addition of iodine, which binds to crystal violet and traps it in the cell
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
Using aseptic technique, prepare and air dried heat fixed slide with the desired organism. Prepare a boiling water bath. Cover the slide with a piece of paper towel and place on staining rack over the water bath. Flood the paper towel on the slide with Malachite Green ( primary stain). Steam the slide for 5 to 7 minutes (mordant).
A stained preparation of Bacillus subtilis showing endospores as green and the vegetative cell as red. The Schaeffer–Fulton stain is a technique designed to isolate endospores by staining any present endospores green, and any other bacterial bodies red. [1]
Carbol fuchsin is applied to a heat-fixed slide. The slide is then heated over a bunsen burner, or suspended over a hot water bath, covered with a paper towel, and steamed for 3 minutes. The slide is rinsed with acidified ethanol, and counter-stained with Methylene blue .
Major applications include blood smears, bone marrow aspirates, semen analysis and cytology of various body fluids including urine and cerebrospinal fluid. [7] [8] Microbiologic agents, such as bacteria and fungi, also appear more easily in Diff-Quik. [3] This is useful for the detection of for example Helicobacter pylori from gastric and ...