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Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
In 1964, Adam S. G. Curtis coined the term Interference Reflection Microscopy (IRM), using it in the field of cell biology to study embryonic chick heart fibroblasts. [ 1 ] [ 2 ] He used IRM to look at adhesion sites and distances of fibroblasts, noting that contact with the glass was mostly limited to the cell periphery and the pseudopodia .
Raman microscopy, and in particular confocal microscopy, can reach down to sub-micrometer lateral spatial resolution. [7] Because a Raman microscope is a diffraction-limited system, its spatial resolution depends on the wavelength of light and the numerical aperture of the focusing element. In confocal Raman microscopy, the diameter of the ...
Bruker Releases New Benchmark 3D Optical Microscope System ContourGT-I Is First Bench Top with Tip/Tilt Head and Vibration Tolerant Design TUCSON, Ariz.--(BUSINESS WIRE)-- Bruker announced the ...
For the first time, scientists have peered into living cells and created videos showing how they function with unprecedented 3D detail. Using a special microscope and new lighting techniques, a ...
When scientists use a traditional microscope to observe a cell, they use stains -- chemicals that color parts of the cell to make them visible. However, these stains cause damage and kill the cell ...
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