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A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology .
Gram-negative bacteria will stain a pink color due to the thin layer of peptidoglycan. If a bacteria stains purple, due to the thick layer of peptidoglycan, the bacteria is a gram-positive bacteria. [4] In clinical microbiology numerous other staining techniques for particular organisms are used (acid fast bacterial stain for mycobacteria).
TSA plates support growth of many semifastidious bacteria, including some species of Brucella, Corynebacterium, Listeria, Neisseria, and Vibrio. Xylose-lysine-deoxycholate agar is used for the culture of stool samples and contains two indicators. It is formulated to inhibit Gram-positive bacteria, while the growth of Gram-negative bacilli is ...
Bacteria in the culture on the right are resistant to most of the antibiotics. In addition to drugs being specific to a certain kind of organism (bacteria, fungi, etc.), some drugs are specific to a certain genus or species of organism, and will not work on other organisms. Because of this specificity, medical microbiologists must consider the ...
Columbia Nalidixic Acid (CNA) agar is a growth medium used for the isolation and cultivation of bacteria from clinical and non-clinical specimens. CNA agar contains antibiotics (nalidixic acid and colistin) that inhibit Gram-negative organisms, aiding in the selective isolation of Gram-positive bacteria. [1]
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
for viral culture detection Hungate Anaerobic tubes: for culturing of anaerobic microbes Incubator: used for bacterial or fungal cultures Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic
Plasmids are almost always purified from liquid bacteria cultures, usually E. coli, which have been transformed and isolated. [5] [6] Virtually all plasmid vectors in common use encode one or more antibiotic resistance genes as a selectable marker, for example a gene encoding ampicillin or kanamycin resistance, which allows bacteria that have been successfully transformed to multiply uninhibited.