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An important factor contributing to the stability of the construct in step I is a high GC-content at the 3' ends and length of the overlap. The third step occurs in the next cycle, when a single strand of the product of step II is used as a template to which fresh primers anneal leading to synthesis of more PD product. [1]
The simplest and oldest one-step method, the explicit Euler method, was published by Leonhard Euler in 1768. After a group of multi-step methods was presented in 1883, Carl Runge, Karl Heun and Wilhelm Kutta developed significant improvements to Euler's method around 1900. These gave rise to the large group of Runge-Kutta methods, which form ...
In modern firearm designs, a firing pin and primer are used to ignite the propellant in the cartridge which propels the bullet forward. The firing pin must travel a short distance, creating a short delay between the user pulling the trigger and the weapon firing, which generally decreases accuracy.
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Multiple journalists thought the video represented YouTube as a whole and stated it was a monumental step for the platform's history. Karim later updated the video's description to criticize YouTube's usage of Google+ accounts and removal of dislikes from public view. As of January 2025, the video has received more than 345 million views. [1]
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The first step to firing a firearm of any sort is igniting the propellant. The earliest firearms were hand cannons, which were simple closed tubes.There was a small aperture, the "touchhole", drilled in the closed end of the tube, leading to the main powder charge.
The first digital watch was the Pulsar, introduced by the Hamilton Watch Company in 1972. The "Pulsar" became a brand name, and would later be acquired by Seiko in 1978. In 1982, a Pulsar watch (NL C01) was released which could store 24 digits, likely making it the first watch with user-programmable memory, or the first "memorybank" watch.