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Different ways of representing a methyl group (highlighted in blue) In organic chemistry, a methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms, having chemical formula CH 3 (whereas normal methane has the formula CH 4). In formulas, the group is often abbreviated as Me.
Methylation, in the chemical sciences, is the addition of a methyl group on a substrate, or the substitution of an atom (or group) by a methyl group. Methylation is a form of alkylation, with a methyl group replacing a hydrogen atom. These terms are commonly used in chemistry, biochemistry, soil science, and biology.
The Schiff reagent is the reaction product of a dye formulation such as fuchsin and sodium bisulfite; pararosaniline (which lacks an aromatic methyl group) and new fuchsin (which is uniformly mono-methylated ortho to the dye's amine functionalities) are not dye alternatives with comparable detection chemistry. In its use as a qualitative test ...
The iodoform reaction tests for the presence of methyl ketones, or compounds which can be oxidized to methyl ketones; The Schiff test detects aldehydes; Tollens' reagent tests for aldehydes (known as the silver mirror test) The Zeisel determination tests for the presence of esters or ethers; Lucas' reagent is used to distinguish between primary ...
MTT, a yellow tetrazole, is reduced to purple formazan in living cells. [8] A solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple formazan product into a colored solution.
Lucas test: negative (left) with ethanol and positive with t-butanol "Lucas' reagent" is a solution of anhydrous zinc chloride in concentrated hydrochloric acid.This solution is used to classify alcohols of low molecular weight.
The term "methyl" was derived in about 1840 by back-formation from "methylene", and was then applied to describe "methyl alcohol". This was shortened to "methanol" in 1892 by the International Conference on Chemical Nomenclature. [29] The suffix-yl, which, in organic chemistry, forms names of carbon groups, is from the word methyl.
Figure 2: Outline of the chemical reaction that underlies the bisulfite-catalyzed conversion of cytosine to uracil. Bisulfite [ 1 ] sequencing (also known as bisulphite sequencing ) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern of methylation .