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conjugative - mediate DNA transfer through conjugation and therefore spread rapidly among the bacterial cells of a population; e.g., F plasmid, many R and some col plasmids. nonconjugative - do not mediate DNA through conjugation, e.g., many R and col plasmids. The pBR322 plasmid is one of the first plasmids widely used as a cloning vector.
Animal Farm is a satirical allegorical novella, in the form of a beast fable, [1] by George Orwell, first published in England on 17 August 1945. [2] [3] It tells the story of a group of anthropomorphic farm animals who rebel against their human farmer, hoping to create a society where the animals can be equal, free, and happy.
The term plasmid was coined in 1952 by the American molecular biologist Joshua Lederberg to refer to "any extrachromosomal hereditary determinant." [14] [15] The term's early usage included any bacterial genetic material that exists extrachromosomally for at least part of its replication cycle, but because that description includes bacterial viruses, the notion of plasmid was refined over time ...
Boxer's strength plays a huge part in keeping Animal Farm together prior to his death: the rest of the animals trusted in it to keep their spirits high during the long and hard laborious winters. Boxer was the only close friend of Benjamin, the cynical donkey. Boxer fights in the Battle of the Cowshed and the Battle of the Windmill.
Squealer is a fictional character, a pig, in George Orwell's 1945 novel Animal Farm.He serves as second-in-command to Napoleon and is the farm's minister of propaganda.He is described in the book as an effective and very convincing orator and a fat porker.
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolivar Zapata, the postdoctoral researcher and Raymond L. Rodriguez. The p stands for "plasmid," and BR for "Bolivar" and "Rodriguez."
The downside, however, is the low yield of product in comparison to prokaryotic vectors as well as the costly nature of the techniques involved. Its complicated technology, and potential contamination with animal viruses of mammalian cell expression have also placed a constraint on its use in large-scale industrial production. [37]
Snowball is a character in George Orwell's 1945 novella Animal Farm.He is largely based on Leon Trotsky, who led the opposition against Joseph Stalin ().Snowball is depicted as an intellectual white pig whose leadership, dedication, and feats for Animal Farm is unparallel to any others on the farm, however he is rivaled by Napoleon who has hatred for Snowball.