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Piezospectroscopy (also known as photoluminescence piezospectroscopy) is an analytical technique that reveals internal stresses in alumina-containing materials, ...
Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase) and a porous solid (the stationary phase).
Photoluminescence (abbreviated as PL) is light emission from any form of matter after the absorption of photons (electromagnetic radiation). [1] It is one of many forms of luminescence (light emission) and is initiated by photoexcitation (i.e. photons that excite electrons to a higher energy level in an atom), hence the prefix photo- . [ 2 ]
With fluorescence correlation spectroscopy, one protein is labeled with a fluorescent dye and the other is left unlabeled. The two proteins are then mixed and the data outputs the fraction of the labeled protein that is unbound and bound to the other protein, allowing you to get a measure of K D and binding affinity. You can also take time ...
The principle of tandem-affinity purification of multiprotein complexes is not limited to the combination of CBP and Protein A tags used in the original work by Rigaut et al. (1999). For example, the combination of FLAG- and HA-tags has been used since 2000 by the group of Nakatani [10] [11] to purify numerous protein complexes from mammalian ...
Fluorescence-lifetime imaging yields images with the intensity of each pixel determined by , which allows one to view contrast between materials with different fluorescence decay rates (even if those materials fluoresce at exactly the same wavelength), and also produces images which show changes in other decay pathways, such as in FRET imaging.
For protein expression and purification in bacteria it is recommended that protein induction and cell harvesting should be done at specific OD 600 (usually at the end of the log phase, OD 600 = 0.4). OD 600 is preferable to UV spectroscopy when measuring the growth over time of a cell population because at this wavelength, the cells will not be ...
The sample surface is a key component in the purification, desorption, and ionization of the sample. SELDI is typically used with time-of-flight (TOF) mass spectrometers and is used to detect proteins in tissue samples, blood , urine , or other clinical samples, however, SELDI technology can potentially be used in any application by simply ...
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