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An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]
Chromobacterium violaceum is a Gram-negative, facultative anaerobic, non-sporing coccobacillus. It is motile with the help of a single flagellum which is located at the pole of the coccobacillus. Usually, there are one or two more lateral flagella as well. [ 1 ]
TSA plates support growth of many semifastidious bacteria, including some species of Brucella, Corynebacterium, Listeria, Neisseria, and Vibrio. Xylose-lysine-deoxycholate agar is used for the culture of stool samples and contains two indicators. It is formulated to inhibit Gram-positive bacteria, while the growth of Gram-negative bacilli is ...
Serratia marcescens (/ s ə ˈ r eɪ ʃ i ə m ɑːr ˈ s ɛ s ɪ n z /) [3] [failed verification] is a species of rod-shaped, Gram-negative bacteria in the family Yersiniaceae. It is a facultative anaerobe and an opportunistic pathogen in humans. It was discovered in 1819 by Bartolomeo Bizio in Padua, Italy. [4]
Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color. The media inhibits growth of gram-positive organisms with crystal violet and bile salts, allowing for the selection and isolation of gram-negative bacteria. The media detects lactose fermentation by enteric bacteria with the pH indicator neutral red. [2]
Gram-negative bacteria are bacteria that, unlike gram-positive bacteria, do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. [1] Their defining characteristic is that their cell envelope consists of a thin peptidoglycan cell wall sandwiched between an inner ( cytoplasmic ) membrane and an outer ...
A. faecalis is a Gram-negative bacterium which appears rod-shaped and motile under a microscope. It is positive by the oxidase test and catalase test, but negative by the nitrate reductase test. It is alpha-hemolytic and requires oxygen. A. faecalis can be grown at 37 °C, and forms colonies that lack pigmentation. [1]
The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.