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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
Cell spreaders. In microbiology, a cell spreader or plate spreader is a tool used to smoothly spread cells and bacteria on a culture plate, such as a petri dish.. Cell spreaders can be made from glass, plastic, or metal, and come in various shapes.
Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter: McCartney's bottle or Flat medical bottle
A close up of an inoculation needle. An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. [1] [full citation needed] It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. [1]
After inoculation and incubation, any gas that is produced will form a visible gas bubble inside the small tube. [1] Litmus solution can also be added to the culture media to give a visual representation of pH changes that occur during the production of gas. [1] [2] The method was first reported in 1898 by British microbiologist Herbert Durham. [1]
In biology, inoculum (pl.: inocula) refers to the source material used for inoculation. Inoculum may refer to: In medicine, material that is the source of the inoculation in a vaccine; In microbiology, propagules: cells, tissue, or viruses that are used to inoculate a new culture
Negative selection through replica plating to screen for ampicillin sensitive colonies. Replica plating is a microbiological technique in which one or more secondary Petri plates containing different solid (agar-based) selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of microorganisms from a primary ...