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Ion chromatography is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger. Learn about the two types of ion chromatography (anion-exchange and cation-exchange), the history of its development, and its applications in water analysis, protein purification, and quality control.
HPLC is a technique to separate, identify, and quantify specific components in mixtures using high pressure pumps and a column filled with adsorbent material. It relies on different interactions between sample components and the adsorbent, which affect their migration rates and retention times in the column.
Chromatography is a technique for separating a mixture into its components by differential partitioning between a mobile and a stationary phase. Learn about its origin, applications, and common terms such as analyte, eluent, chromatogram, and retention time.
Ion exchange is a reversible interchange of ions between a solid and a solution. It is used for water treatment, chemical purification, biomolecule separation, and more. Learn about the different types of ion exchangers, resins, and chromatography methods.
Size-exclusion chromatography (SEC) is a method to separate molecules by their shape and size using porous beads in a column. It is also known as gel-filtration chromatography or gel permeation chromatography depending on the solvent used.
Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography for protein and nucleic acid separation. Learn about its preparation, types, applications, and ion-exchange mechanism.
Anion exchange chromatography is a technique that separates substances based on their charges using a positively charged resin, such as DEAE. It is used to purify proteins, amino acids, sugars and other acidic substances.
A chromatographic technique that uses polar stationary phases and aqueous mobile phases with varying polarity. Learn about the principle, features, and applications of aqueous normal-phase chromatography (ANP) and its distinction from normal-phase and reversed-phase chromatography.