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Bone marrow samples can be obtained by aspiration and trephine biopsy. Sometimes, a bone marrow examination will include both an aspirate and a biopsy. The aspirate yields semi-liquid bone marrow, which can be examined by a pathologist under a light microscope and analyzed by flow cytometry, chromosome analysis, or polymerase chain reaction (PCR
Examples are thoracocentesis to sample pleural fluid, and amniocentesis to sample amniotic fluid. The main method of centesis, in turn, is fine needle aspiration, but there are also somewhat differently designed needles, such as for bone marrow aspiration. Puncture without aspiration may suffice in, for example, capillary blood sampling.
Some specimen types also require special treatment, such as immediate mixture with an additive, or storage at a certain temperature. [5] After extraction , all specimen containers must be labeled with at least two of the following identifiers (at the time of collection): patient's name, date of birth, hospital number, test request form number ...
used for taking a biopsy from a deep hard tissue like bone marrow (within a hard bone) Spirometer: used to test lung function; video link •Water-seal type-do- •Douglas bag type-do- Peak flow meter or peak expiatory flow rate meter: used to test lung function by testing the rate at which the person can exhale; useful to diagnose COPD and asthma
Bone marrow is a semi-solid tissue found within the spongy (also known as cancellous) portions of bones. [2] In birds and mammals, bone marrow is the primary site of new blood cell production (or haematopoiesis). [3] It is composed of hematopoietic cells, marrow adipose tissue, and supportive stromal cells.
The Jamshidi needle is a trephine needle for performing bone marrow biopsy, whereby a cylindrical sample of tissue, a core biopsy specimen, is obtained. It is a cylindrical needle with a tapered cutting tip. [1] The tapered end reduces the potential of crush artifact. [2] It is the most commonly used needle for performing bone marrow biopsies. [3]
Once the target is determined, a sample of blood or bone marrow is obtained, nucleic acid is extracted, and the sample analyzed for the leukaemic sequence. These tests are very specific, and detect leukaemic cells at levels down to one cell in a million, though the limit typically achieved is one in 10,000 to one in 100,000 cells.
Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates, which are examined under a light microscope.