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Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
Beta-gal is inhibited by L-ribose and by competitive inhibitors 2-phenylethyl 1-thio-β-D-galactopyranoside (PETG), D-galactonolactone, isopropyl thio-β-D-galactoside (IPTG), and galactose. [ 4 ] β-Galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of ...
Women are 4-5 times more likely to develop a clot during pregnancy and in the postpartum period than when they are not pregnant. [25] Hypercoagulability in pregnancy likely evolved to protect women from hemorrhage at the time of miscarriage or childbirth. In developing countries, the leading cause of maternal death is still hemorrhage. [25]
The Autoimmune Protocol (AIP) diet is an elimination diet designed to help reduce inflammation, manage symptoms and improve quality of life for people with autoimmune diseases.
The tattooed corpse of a woman was found bizarrely stuffed in a refrigerator dumped in some New Jersey woods — and cops say they need the public’s help identifying her.
Women who become pregnant after age 35 are at increased risk for complications that affect the mother and fetus. When it comes to the mother, several research studies have shown that pregnant women over 35 years of age are at increased risk for hypertension during pregnancy , eclampsia (hypertension during pregnancy with seizures), and ...
In this video, we meet Peaches, an average barn cat who doesn’t mind blowing off work to chill with her BFF, a senior horse.Though Peaches was adopted and given a home in this family’s barn to ...