Search results
Results from the WOW.Com Content Network
CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression of gene expression in prokaryotic and eukaryotic cells. [1] It was first developed by Stanley Qi and colleagues in the laboratories of Wendell Lim , Adam Arkin, Jonathan Weissman , and Jennifer Doudna . [ 2 ]
Apart from knock-out there are also knock-down (CRISPRi) and activation (CRISPRa) libraries, which use the ability of proteolytically deactivated Cas9-fusion proteins (dCas9) to bind target DNA, which means that a gene of interest is not cut but is over-expressed or repressed. It made CRISPR/Cas9 system even more interesting in gene editing.
CRISPR interference (CRISPRi) on the other hand utilizes a catalytically inactive nuclease to physically block RNA polymerase, effectively preventing or halting transcription. [8] Perturb-seq has been utilized with both the knockout and CRISPRi approaches in the Dixit et al. paper [2] and the Adamson et al. paper, [1] respectively.
Main page; Contents; Current events; Random article; About Wikipedia; Contact us
The exact protocol for lentiviral production will vary depending on the research aim and applied library. [35] [43] [44] If a two vector-system is used, for example, cells are sequentially transduced with Cas9 and sgRNA in a two-step procedure. [35] [44] Although more complex, this has the advantage of a higher titre for the sgRNA library virus ...
The Oxford Dictionary of English (ODE) is a single-volume English dictionary published by Oxford University Press, first published in 1998 as The New Oxford Dictionary of English (NODE). The word "new" was dropped from the title with the Second Edition in 2003. [ 1 ]
The Oxford English Dictionary (OED) is the principal historical dictionary of the English language, published by Oxford University Press (OUP), a University of Oxford publishing house. The dictionary, which published its first edition in 1884, traces the historical development of the English language, providing a comprehensive resource to ...
The Oxford Advanced Learner's Dictionary (OALD) was the first advanced learner's dictionary of English. It was first published in 1948. It is the largest English-language dictionary from Oxford University Press aimed at a non-native audience.