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  2. Amplified fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Amplified_fragment_length...

    Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by KeyGene, [1] AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction ...

  3. Ligation (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Ligation_(molecular_biology)

    Ligation is complicated by the fact that the reaction can involve both inter- and intra-molecular reactions, but the desired ligation products in many ligation reactions (e.g. ligating a DNA fragment into a vector) needed first to be inter-molecular, i.e. between two different DNA molecules, followed by an intra-molecular reaction to seal and ...

  4. Molecular-weight size marker - Wikipedia

    en.wikipedia.org/wiki/Molecular-weight_size_marker

    There are two common methods in which to construct a DNA molecular-weight size marker. [3] One such method employs the technique of partial ligation. [3] DNA ligation is the process by which linear DNA pieces are connected to each other via covalent bonds; more specifically, these bonds are phosphodiester bonds. [4]

  5. DNA ligase - Wikipedia

    en.wikipedia.org/wiki/DNA_ligase

    DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA).

  6. Ligase - Wikipedia

    en.wikipedia.org/wiki/Ligase

    For example, DNA ligase can join two complementary fragments of nucleic acid by forming phosphodiester bonds, and repair single stranded breaks that arise in double stranded DNA during replication. In general, a ligase catalyzes the following dehydration reaction, thus joining molecules A and B:

  7. DNA ligase 1 - Wikipedia

    en.wikipedia.org/wiki/DNA_ligase_1

    DNA ligase 1 functions to ligate single stranded DNA breaks in the final step of the base excision repair (BER) pathway. [14] The nitrogenous bases of DNA are commonly damaged by environmental hazards such as reactive oxygen species, toxins, and ionizing radiation. BER is the major repair pathway responsible for excising and replacing damaged ...

  8. Sequencing by ligation - Wikipedia

    en.wikipedia.org/wiki/Sequencing_by_ligation

    DNA ligase is an enzyme that joins together ends of DNA molecules. Although commonly represented as joining two pairs of ends at once, as in the ligation of restriction enzyme fragments, ligase can also join the ends on only one of the two strands (for example, when the other strand is already continuous or lacks a terminal phosphate necessary for ligation).

  9. Ligase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Ligase_chain_reaction

    The ligase chain reaction (LCR) is a method of DNA amplification. The ligase chain reaction (LCR) is an amplification process that differs from polymerase chain reaction (PCR) in that it involves a thermostable ligase to join two probes or other molecules together which can then be amplified by standard PCR cycling. [1]

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