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BASys (Bacterial Annotation System) is a tool for automated annotation of bacterial genomic (chromosomal and plasmid) sequences including gene/protein names, GO functions, COG functions, possible paralogues and orthologues, molecular weights, isoelectric points, operon structures, subcellular localization, signal peptides, transmembrane regions ...
The LOC100287387 protein is formed by a 423 amino acid peptide sequence. The molecular mass is 44.4 kdal, [ 5 ] and the isoelectric point is 10.77. [ 6 ] There is a G-patch domain and a short domain of unknown function within the peptide sequence.
The two dimensions that proteins are separated into using this technique can be isoelectric point, protein complex mass in the native state, or protein mass. [citation needed] The separation by isoelectric point is called isoelectric focusing. Thereby, a pH gradient is applied to a gel and an electric potential is applied across the gel, making ...
A number of algorithms for estimating isoelectric points of peptides and proteins have been developed. Most of them use Henderson–Hasselbalch equation with different pK values. For instance, within the model proposed by Bjellqvist and co-workers, the pKs were determined between closely related immobilines by focusing the same sample in ...
The three samples are mixed and loaded onto IEF (isoelectric focusing chromatography) for first dimension and the strip is transferred to a SDS PAGE.After the gel electrophoresis, the gel is scanned with the excitation wavelength of each dye one after the other, so each sample can be seen separately (if we scan the gel at the excitation wavelength of the Cy3 dye, we will see in the gel only ...
Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). [ 1 ] [ 2 ] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the ...
[15] [16] The protein product of FAM214A consists of 1076 amino acids (aa), has been predicted to have a molecular mass of 121,700 daltons, and has an isoelectric point around pH 7.7. [ 6 ] [ 17 ] [ 18 ] This protein is predicted to remain in the nucleus after transcription based upon its lack of signal peptide sequence and the predictions of ...
The protein product is the coiled-coil domain-containing protein 29, abbreviated CCDC29, is about 291 amino acids in length and contains the DUF3496 motif. Also, this protein is also predicted to have a possible transmembrane site at about 284 - 291 aa. [4] Isoelectric Point:6.043 [4] Molecular Weight 33.6 kdal [4]