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Giemsa stained Trypanosoma parasites (Chagas disease pathogen) Whirling disease section stained with Giemsa stain. Giemsa stain (/ ˈ ɡ iː m z ə /), named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites.
Brugia timori is a filarial (arthropod-borne) nematode (roundworm) which causes the disease "Timor filariasis", or "Timorian filariasis".While this disease was first described in 1965, [1] the identity of Brugia timori as the causative agent was not known until 1977. [2]
Leishmania tropica is a flagellate parasite and the cause of anthroponotic [dubious – discuss] cutaneous leishmaniasis in humans. [2] This parasite is restricted to Afro-Eurasia and is a common cause of infection in Afghanistan, Iran, Syria, Yemen, Algeria, Morocco, and northern India. [3]
Whole blood with microfilaria worm, giemsa stain. L. loa worms have a simple structure consisting of a head (which lacks lips), a body, and a blunt tail. The outer body of the worm is composed of a cuticle with three main layers made up of collagen and other compounds which aid in protecting the nematodes while they are inside the digestive system of their host.
These morphologic changes, referred to as Schüffner's dots, are important in the identification of this species of malarial parasite and have been associated by electron microscopy with caveolavesicle complexes along the erythrocyte plasmalemma. [3] They are named for Wilhelm Schüffner, who described them in 1904. [4]
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Plasmodium ovale microgametocyte in Giemsa-stained thin blood film, with annotated Schüffner's dots and hemozoin pigment. The microscopic appearance of P. ovale is very similar to that of P. vivax and if there are only a small number of parasites seen, it may be impossible to distinguish the two species on morphological grounds alone.
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