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An optical instrument is a device that processes light waves (or photons), either to enhance an image for viewing or to analyze and determine their characteristic properties. Common examples include periscopes, microscopes, telescopes, and cameras. [1] [2]
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Visible light (and near-infrared light) is typically absorbed and emitted by electrons in molecules and atoms that move from one energy level to another. This action allows the chemical mechanisms that underlie human vision and plant photosynthesis. The light that excites the human visual system is a very small portion of the electromagnetic ...
The wavelength of visible light waves varies between 400 and 700 nm, but the term "light" is also often applied to infrared (0.7–300 μm) and ultraviolet radiation (10–400 nm). The wave model can be used to make predictions about how an optical system will behave without requiring an explanation of what is "waving" in what medium.
Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...
By recording the attenuation of light for various wavelengths, an absorption spectrum can be obtained. In physics , absorption of electromagnetic radiation is how matter (typically electrons bound in atoms ) takes up a photon 's energy —and so transforms electromagnetic energy into internal energy of the absorber (for example, thermal energy ).
Light field microscopy (LFM) is a scanning-free 3-dimensional (3D) microscopic imaging method based on the theory of light field.This technique allows sub-second (~10 Hz) large volumetric imaging ([~0.1 to 1 mm] 3) with ~1 μm spatial resolution in the condition of weak scattering and semi-transparence, which has never been achieved by other methods.
At this distance the third medium overlaps the evanescent field, disrupting the total reflection of light in the first medium and allowing propagation of the wave in the third medium. This process is analogous to quantum tunneling; the photons confined within the first medium are able to tunnel through the second medium (where they cannot exist ...
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