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  2. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.

  3. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...

  4. Dark-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Dark-field_microscopy

    Diagram illustrating the light path through a dark-field microscope. The steps are illustrated in the figure where an inverted microscope is used. Light enters the microscope for illumination of the sample. A specially sized disc, the patch stop (see figure), blocks some light from the light source, leaving an outer ring of illumination. A wide ...

  5. Microscopy with UV surface excitation - Wikipedia

    en.wikipedia.org/wiki/Microscopy_with_UV_surface...

    The microscope setup is based on an inverted microscope design. [ 2 ] [ 3 ] [ 4 ] An automated stage is used to record larger areas by mosaicing a series of single adjacent frames. The LED light is focused using a ball lens with a short focal length onto the sample surface in an oblique-angle cis-illumination scheme since standard microscopy ...

  6. Phase-contrast microscopy - Wikipedia

    en.wikipedia.org/wiki/Phase-contrast_microscopy

    When light waves travel through a medium other than a vacuum, interaction with the medium causes the wave amplitude and phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors.

  7. Bright-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Bright-field_microscopy

    The light path of a bright-field microscope is extremely simple; no additional components are required beyond the normal light-microscope setup. The light path begins at the illuminator or the light source on the base of the microscope. Often a halogen lamp is used. The light travels through the objective lens into the ocular lens, through ...

  8. Light field microscopy - Wikipedia

    en.wikipedia.org/wiki/Light_field_microscopy

    Light field microscopy (LFM) is a scanning-free 3-dimensional (3D) microscopic imaging method based on the theory of light field.This technique allows sub-second (~10 Hz) large volumetric imaging ([~0.1 to 1 mm] 3) with ~1 μm spatial resolution in the condition of weak scattering and semi-transparence, which has never been achieved by other methods.

  9. Correlative light-electron microscopy - Wikipedia

    en.wikipedia.org/wiki/Correlative_light-electron...

    Overlay of the two images is thus performed automatically as a result of the integration of two microscopes. [1] This technique is used in order to obtain information at different length scales: the electron microscope provides high-resolution information down to the nano-scale, while the fluorescence microscope highlights the regions of interest.

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