Search results
Results from the WOW.Com Content Network
In chemistry, a salt bridge is a combination of two non-covalent interactions: hydrogen bonding and ionic bonding (Figure 1). Ion pairing is one of the most important noncovalent forces in chemistry, in biological systems, in different materials and in many applications such as ion pair chromatography .
In electrochemistry, a salt bridge or ion bridge is an essential laboratory device discovered over 100 years ago. [ 1 ] It contains an electrolyte solution, typically an inert solution, used to connect the oxidation and reduction half-cells of a galvanic cell (voltaic cell), a type of electrochemical cell .
Bonding energies are significant, with solution-phase values falling within the same order of magnitude as hydrogen bonds and salt bridges. Similar to these other non-covalent bonds, cation–π interactions play an important role in nature, particularly in protein structure, molecular recognition and enzyme catalysis. The effect has also been ...
Salt bridge (protein and supramolecular) Information on intermolecular forces is obtained by macroscopic measurements of properties like viscosity , pressure, volume, temperature (PVT) data. The link to microscopic aspects is given by virial coefficients and intermolecular pair potentials , such as the Mie potential , Buckingham potential or ...
A hydrogen bond (H-bond), is a specific type of interaction that involves dipole–dipole attraction between a partially positive hydrogen atom and a highly electronegative, partially negative oxygen, nitrogen, sulfur, or fluorine atom (not covalently bound to said hydrogen atom). It is not a covalent bond, but instead is classified as a strong ...
Equilibrium constants in water indicate additive free energy contributions for each salt bridge. Another method for the identification of hydrogen bonds also in complicated molecules is crystallography, sometimes also NMR-spectroscopy. The attractive forces defining the strength of ionic bonding can be modeled by Coulomb's Law.
Upgrade to a faster, more secure version of a supported browser. It's free and it only takes a few moments:
A chaotrope denatures biomolecules by disrupting the shell of hydration around them. This allows positively charged ions to form a salt bridge between the negatively charged silica and the negatively charged DNA backbone in high salt concentration. The DNA can then be washed with high salt and ethanol, and ultimately eluted with low salt.