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  2. Elution - Wikipedia

    en.wikipedia.org/wiki/Elution

    Elution then is the process of removing analytes from the adsorbent by running a solvent, called an "eluent", past the adsorbent–analyte complex. As the solvent molecules "elute", or travel down through the chromatography column, they can either pass by the adsorbent–analyte complex or displace the analyte by binding to the adsorbent in its ...

  3. Thermoresponsive polymers in chromatography - Wikipedia

    en.wikipedia.org/wiki/Thermoresponsive_polymers...

    Thermally related benefits of gas chromatography can now be applied to classes of compounds that are restricted to liquid chromatography due to their thermolability. In place of solvent gradient elution, thermoresponsive polymers allow the use of temperature gradients under purely aqueous isocratic conditions. [ 2 ]

  4. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    In isocratic elution, the retention order does not change if the column dimensions (length and inner diameter) change – that is, the peaks elute in the same order. In gradient elution, however, the elution order may change as the dimensions or flow rate change. if they are no scaled down or up according to the change [40]

  5. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Pyrolysis–gas chromatography–mass spectrometry is a method of chemical analysis in which the sample is heated to decomposition to produce smaller molecules that are separated by gas chromatography and detected using mass spectrometry. Pyrolysis is the thermal decomposition of materials in an inert atmosphere or a vacuum.

  6. Headspace gas chromatography for dissolved gas measurement

    en.wikipedia.org/wiki/Headspace_Gas...

    As such, headspace gas chromatography offers a method for determining if there is natural biodegradation processes occurring in contaminated aquifers. [5] For example, fuel hydrocarbons will break down into methane. Chlorinated solvents such as trichloroethylene, break down into ethene and chloride.

  7. Gas chromatography - Wikipedia

    en.wikipedia.org/wiki/Gas_chromatography

    Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture. [ 1 ]

  8. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    The organic solvent is called also a modifier, since it is added to the aqueous solution in the mobile phase in order to modify the polarity of the mobile phase. Water is the most polar solvent in the reversed phase mobile phase; therefore, lowering the polarity of the mobile phase by adding modifiers enhances its elution strength.

  9. Displacement chromatography - Wikipedia

    en.wikipedia.org/wiki/Displacement_chromatography

    Resolution in elution chromatography is generally better when peaks are strongly retained, but conditions that give good resolution of early peaks lead to long run-times and excessive broadening of later peaks unless gradient elution is employed. Gradient equipment adds complexity and expense, particularly at large scale.