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Molecular breeding is the application of molecular biology tools, often in plant breeding [1] [2] and animal breeding. [3] [4] In the broad sense, molecular breeding can be defined as the use of genetic manipulation performed at the level of DNA to improve traits of interest in plants and animals, and it may also include genetic engineering or gene manipulation, molecular marker-assisted ...
DNA shuffling by molecular breeding was first reported in 1994 by Willem P.C. Stemmer. [1] [7] He started by fragmenting the β-lactamase gene that had been amplified with the polymerase chain reaction (PCR) by using DNase I, which randomly cleaves DNA.
[1]: 1 Various techniques were developed to aid in breeding and selection. Hybridization was one way rapid changes in an organism's genetic makeup could be introduced. Crop hybridization most likely first occurred when humans began growing genetically distinct individuals of related species in close proximity.
This is an accepted version of this page This is the latest accepted revision, reviewed on 18 December 2024. Manipulation of an organism's genome For a non-technical introduction to the topic of genetics, see Introduction to genetics. For the song by Orchestral Manoeuvres in the Dark, see Genetic Engineering (song). For the Montreal hardcore band, see Genetic Control. Part of a series on ...
Other molecular breeding tool include development of genetically modified crops that can tolerate plant stress. As a complement to the recent rapid progress in genomics , a better understanding of physiological mechanisms of dehydration response will also contribute to the progress of genetic enhancement of crop drought resistance.
A sequence related amplified polymorphism (SRAP) is a molecular technique, developed by G. Li and C. F. Quiros in 2001, [1] for detecting genetic variation in the open reading frames (ORFs) of genomes of plants and related organisms.
Nested association mapping (NAM) is a technique designed by the labs of Edward Buckler, James Holland, and Michael McMullen for identifying and dissecting the genetic architecture of complex traits in corn ().
Dr. Singh has published more than 120 research articles in international and national journals on rice genetics, molecular breeding and grain quality. Marker Assisted Plant Breeding: Principles and Practices [6] is a book he co-authored with Professor Brahma Deo Singh and published by Springer. A complete list of Dr Singh's publications is ...