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  2. Primary cell culture - Wikipedia

    en.wikipedia.org/wiki/Primary_cell_culture

    Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]

  3. Cell isolation - Wikipedia

    en.wikipedia.org/wiki/Cell_isolation

    Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells ), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.

  4. Laser capture microdissection - Wikipedia

    en.wikipedia.org/wiki/Laser_capture_microdissection

    Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations.

  5. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and ...

  6. Immunomagnetic separation - Wikipedia

    en.wikipedia.org/wiki/Immunomagnetic_separation

    Another laboratory separation tool is the affinity magnetic separation (AMS), which is more suitable for the isolation of prokaryotic cells. [2] IMS deals with the isolation of cells, proteins, and nucleic acids through the specific capture of biomolecules through the attachment of small-magnetized particles, beads, containing antibodies and ...

  7. Single-cell analysis - Wikipedia

    en.wikipedia.org/wiki/Single-cell_analysis

    This single cell shows the process of the central dogma of molecular biology, which are all steps researchers are interested to quantify (DNA, RNA, and Protein).. In cell biology, single-cell analysis and subcellular analysis [1] refer to the study of genomics, transcriptomics, proteomics, metabolomics, and cellcell interactions at the level of an individual cell, as opposed to more ...

  8. It's the end of the road for these cars: Models that won't ...

    www.aol.com/end-road-cars-models-wont-131806139.html

    A number of car models won't ring in the new year.. The Ford Edge, Toyota Venza and Mini Clubman are just some of the vehicles that won't make it past model year 2024 in U.S. markets.

  9. Acid guanidinium thiocyanate-phenol-chloroform extraction

    en.wikipedia.org/wiki/Acid_guanidinium...

    This method may take longer than a column-based system such as the silica-based purification, but has higher purity and the advantage of high recovery of RNA. [1] Furthermore, an RNA column is typically unsuitable for purification of short (<200 nucleotides ) RNA species, such as siRNA , miRNA and tRNA .

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