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The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols , hydroxamic acids , oximes, and sulfinic acids give positive results as well. [ 1 ]
To calculate phenol coefficient, the concentration of phenol at which the compound kills the test organism in 10 minutes, but not in 5 minutes, is divided by the concentration of the test compound that kills the organism under the same conditions (or, probably more common, dividing the dilution factor at which the tested substance shows ...
It is the ratio of the dilution of the disinfectant that kills a microorganism to the dilution of phenol that kills the organism in the same time under identical conditions. The Rideal–Walker coefficient determines the phenol coefficient utilizing the method (test) described by English chemists Samuel Rideal (1863–1929) and J. T. Ainslie ...
Berthelot's reagent is an alkaline solution of phenol and hypochlorite, used in analytical chemistry. It is named after its inventor, Marcellin Berthelot. Ammonia reacts with Berthelot's reagent to form a blue product which is used in a colorimetric method for determining ammonia. The reagent can also be used for determining urea.
Phenol is also a versatile precursor to a large collection of drugs, most notably aspirin but also many herbicides and pharmaceutical drugs. Phenol is a component in liquid–liquid phenol–chloroform extraction technique used in molecular biology for obtaining nucleic acids from tissues or cell culture samples.
The Folin–Ciocâlteu reagent (FCR) or Folin's phenol reagent or Folin–Denis reagent, is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants, also called the gallic acid equivalence method (GAE). [1] It is named after Otto Folin, Vintilă Ciocâlteu, and Willey ...
Ferric chloride (FeCl 3) test is also a colorimetric assay. Lamaison and Carnet have designed a test for the determination of the total flavonoid content of a sample (AlCI 3 method). After proper mixing of the sample and the reagent, the mixture is incubated for 10 minutes at ambient temperature and the absorbance of the solution is read at 440 nm.
The acid-fast staining method, in conjunction with auramine phenol staining, serves as the standard diagnostic tool and is widely accessible for rapidly diagnosing tuberculosis (caused by Mycobacterium tuberculosis) and other diseases caused by atypical mycobacteria, such as leprosy (caused by Mycobacterium leprae) and Mycobacterium avium ...