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Radiation hybrid mapping consists of several steps. A radiation hybrid is "a cell or organism that contains fragments of chromosomes from a second organism". [ 2 ] : 82 Radiation hybrids are generated by using X-rays to randomly break chromosomes into fragments, then implanting the fragments into non-irradiated rodent cells, which replicate and ...
In genomics, DNA–DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between DNA sequences. It is used to determine the genetic distance between two organisms and has been used extensively in phylogeny and taxonomy .
8–12μl of each of labelled test and labelled reference DNA are mixed and 40 μg Cot-1 DNA is added, then precipitated and subsequently dissolved in 6μl of hybridization mix, which contains 50% formamide to decrease DNA melting temperature and 10% dextran sulphate to increase the effective probe concentration in a saline sodium citrate (SSC ...
A hybrid between a sulcata tortoise (Centrochelys sulcata) and leopard tortoise (Stigmochelys pardalis). Family Emydidae. Subfamily Deirochelyinae. Hybrid between red-eared slider and Ouachita map turtle. Genus Trachemys. Species T. scripta. The hybrid between a red-eared slider and a yellow-bellied slider. Class Mammalia. Clade Euungulata ...
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The Caspian red deer's winter coat is greyer and has a larger and more distinguished light rump-patch (like wapiti and some central Asian red deer) compared with the Western European red deer, which has more of a greyish-brown coat with a darker yellowish rump patch in the winter.
The judge in Sean "Diddy" Combs' sex-trafficking case has questions about the rap mogul's notepads. Someone wrote "Legal" on the pads in the days after prison officials took evidence photos of them.
Colony hybridization is a method of selecting bacterial colonies with desired genes through a straightforward cloning and transfer process. [1] The genes of interest have been added to a bacterial plasmid previously through recombination , allowing genes from other organisms to be analyzed within a bacterial colony.