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New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. [2] It also provides products and services supporting genome editing, synthetic biology and next-generation sequencing. [3]
Schematic overview of the MERCURIUS BRB-seq workflow where up to 384 samples can be barcoded and multiplexed per kit.. Bulk RNA barcoding and sequencing (BRB-seq) is an ultra-high-throughput bulk 3' mRNA-seq technology that uses early-stage sample barcoding and unique molecular identifiers (UMIs) to allow the pooling of up to 384 samples in one tube early in the sequencing library preparation ...
As part of the Gene Alliance, founded in 1998, the company was involved in the large-scale genome analysis project Aspergillus niger funded by DSM (The Netherlands) and finished in 2001. [3] In 2006 the company started to develop the next generation sequencing business division adding the Europe's first commercial PacBio RS platform in 2011.
The company provides a line of products and services that serves the sequencing, genotyping and gene expression, and proteomics markets, and serves more than 155 countries. [2] Illumina's customers include genomic research centers, pharmaceutical companies, academic institutions, clinical research organizations, and biotechnology companies. [1]
DNA sequencing with commercially available NGS platforms is generally conducted with the following steps. First, DNA sequencing libraries are generated by clonal amplification by PCR in vitro . Second, the DNA is sequenced by synthesis , such that the DNA sequence is determined by the addition of nucleotides to the complementary strand rather ...
Tempus AI, Inc. is an American health technology company [1] founded in 2015 by Eric Lefkofsky in Chicago, Illinois. [2] It was established by Lefkofsky soon after his wife was diagnosed with breast cancer. [3]
RNA-Seq was first developed in mid 2000s with the advent of next-generation sequencing technology. [144] The first manuscripts that used RNA-Seq even without using the term includes those of prostate cancer cell lines [ 145 ] (dated 2006), Medicago truncatula [ 146 ] (2006), maize [ 147 ] (2007), and Arabidopsis thaliana [ 148 ] (2007), while ...
In 2019, they published their new single-tube long fragment read (stLFR) technology, enabling the construction of long DNA molecules from short reads using a combinatorial process of DNA barcoding. It enables phasing, SV detection, scaffolding, and cost-effective diploid de novo genome assembly from second-generation sequencing technology. [20]
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