Ads
related to: gene sequencing methodsthetech.org has been visited by 10K+ users in the past month
Search results
Results from the WOW.Com Content Network
DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA.It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine.
Whereas the methods above describe various sequencing methods, separate related terms are used when a large portion of a genome is sequenced. Several platforms were developed to perform exome sequencing (a subset of all DNA across all chromosomes that encode genes) or whole genome sequencing (sequencing of the all nuclear DNA of a human).
Common challenges of DNA sequencing with the Sanger method include poor quality in the first 15–40 bases of the sequence due to primer binding and deteriorating quality of sequencing traces after 700–900 bases. Base calling software such as Phred typically provides an estimate of quality to aid in trimming of low-quality regions of sequences.
The first DNA sequencing methods were developed by Gilbert (1973) [8] and Sanger (1975). [9] Gilbert introduced a sequencing method based on chemical modification of DNA followed by cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased ...
A method particularly suitable for the discovery of genomic structural variation is Single-cell DNA template strand sequencing (a.k.a. Strand-seq). [17] Using the principle of single-cell tri-channel processing, which uses joint modelling of read-orientation, read-depth, and haplotype-phase, Strand-seq enables discovery of the full spectrum of ...
The DNA sequencing methods used in the 1970s and 1980s were manual; for example, Maxam–Gilbert sequencing and Sanger sequencing. Several whole bacteriophage and animal viral genomes were sequenced by these techniques, but the shift to more rapid, automated sequencing methods in the 1990s facilitated the sequencing of the larger bacterial and ...
Ads
related to: gene sequencing methodsthetech.org has been visited by 10K+ users in the past month