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  2. DNA sequencing - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencing

    DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and ...

  3. Whole genome sequencing - Wikipedia

    en.wikipedia.org/wiki/Whole_genome_sequencing

    Whole genome sequencing (WGS) is the process of determining the entirety, or nearly the entirety, of the DNA sequence of an organism's genome at a single time. [2] This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.

  4. Sanger sequencing - Wikipedia

    en.wikipedia.org/wiki/Sanger_sequencing

    Microfluidic Sanger sequencing is a lab-on-a-chip application for DNA sequencing, in which the Sanger sequencing steps (thermal cycling, sample purification, and capillary electrophoresis) are integrated on a wafer-scale chip using nanoliter-scale sample volumes. This technology generates long and accurate sequence reads, while obviating many ...

  5. Exome sequencing - Wikipedia

    en.wikipedia.org/wiki/Exome_sequencing

    Exome sequencing workflow: part 1. Exome sequencing, also known as whole exome sequencing (WES), is a genomic technique for sequencing all of the protein-coding regions of genes in a genome (known as the exome). [1] It consists of two steps: the first step is to select only the subset of DNA that encodes proteins.

  6. RNA-Seq - Wikipedia

    en.wikipedia.org/wiki/RNA-Seq

    The general steps to prepare a complementary DNA (cDNA) library for sequencing are described below, but often vary between platforms. [10] [3] [11] RNA Isolation: RNA is isolated from tissue and mixed with Deoxyribonuclease (DNase). DNase reduces the amount of genomic DNA.

  7. Massive parallel sequencing - Wikipedia

    en.wikipedia.org/wiki/Massive_parallel_sequencing

    DNA sequencing with commercially available NGS platforms is generally conducted with the following steps. First, DNA sequencing libraries are generated by clonal amplification by PCR in vitro . Second, the DNA is sequenced by synthesis , such that the DNA sequence is determined by the addition of nucleotides to the complementary strand rather ...

  8. Sequencing - Wikipedia

    en.wikipedia.org/wiki/Sequencing

    DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates.

  9. Single-cell sequencing - Wikipedia

    en.wikipedia.org/wiki/Single-cell_sequencing

    The final step of the platform is the sequencing. Libraries generated can be directly used for single cell whole transcriptome sequencing or target sequencing workflows. The sequencing is performed by using the Illumina dye sequencing method. This sequencing method is based on sequencing by synthesis (SBS) principle and the use of reversible ...

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